NKp80 stimulates alloresponses of CD8 T cells toward macrophages. (A,B) Purified CD8+ T cells were stimulated with irradiated allogeneic macrophages for 8 days in the presence of anti-NKp80 (Fab′)2 5D12, or control IgG1 (stimulation) and subsequently, assayed for IFNγ secretion upon restimulation with macrophages. Allogeneic macrophages used for stimulation and restimulation came from the same culture. Dot plots (A) and mean and standard deviations of triplicates (B) for one representative of 3 experiments are shown. (C,D) CFSE-labeled, purified CD8 T cells were cocultured with allogeneic macrophages for 8 days in the presence of anti-NKp80 (Fab′)2 5D12 or control IgG1 and frequencies of proliferating cells analyzed by flow cytometry. Histogram plots (C) and means and standard deviations of triplicates (D) for one representative of 3 experiments are shown. Purified CD8 T cells used for experiments shown in panels A through D were approximately 15%-20% NKp80+.
