Id2 regulates erythroid development in vivo. (A) BMC-GFP and BMC-Id2-GFP were transplanted into lethally irradiated mice with supporting BMCs. A representative from 4 independent experiments was shown here. Eight mice were analyzed in each transplantation. Erythroid reconstitution in recipient BM was examined 4 weeks after transplantation by FACS analysis of GFP-positive cells using TER119 and CD71 antibodies. (B) BMC-NS-shRNA and BMC-Id2-shRNA were transplanted into lethally irradiated mice with supporting BMCs. A representative from 3 independent experiments is shown here. Eight mice were analyzed in each transplantation. Erythroid reconstitution was examined 4 weeks after transplantation in recipient BM by FACS analysis using TER119 and CD71 antibodies.