Gene expression signatures of DCs as a function of loading methodology. (A) Signatures of the top 100 most differentially expressed genes. Duplicate results shown. Unloaded, unloaded DCs; mRNA, DC loaded by mRNA electroporation; lysate, DC loaded by incubation with cell lysates; mismatch, DC doubly loaded with mRNA/lysate preparations derived from disparate cell types; both, DC doubly loaded with mRNA/lysate preparations derived from same cell type. (B) Signatures of all 1750 differentially regulated genes.