Figure 5
Figure 5. Dexamethasone induced apoptosis and NF-κB transrepression in different myeloma cell lines. U266, Opm-2, 8226, ANBL-6, AF-10, and ARH77 cell lines were treated with 10−6 M dexamethasone for 72 hours and percentage of cells undergoing apoptosis identified by caspase 3 assay. Bar diagram shows percentage of cells undergoing apoptosis (■). NF-κB transrepression experiment was performed as described in “Reporter assays.” □ shows the percentage decrease in the NF-κB reporter activity on the addition of dexamethasone. Both the experiments were done twice with similar results.

Dexamethasone induced apoptosis and NF-κB transrepression in different myeloma cell lines. U266, Opm-2, 8226, ANBL-6, AF-10, and ARH77 cell lines were treated with 10−6 M dexamethasone for 72 hours and percentage of cells undergoing apoptosis identified by caspase 3 assay. Bar diagram shows percentage of cells undergoing apoptosis (■). NF-κB transrepression experiment was performed as described in “Reporter assays.” □ shows the percentage decrease in the NF-κB reporter activity on the addition of dexamethasone. Both the experiments were done twice with similar results.

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