Committed progenitors from Ptpn11^D61Y mice evoke CICs. (A) Ptpn11^D61Y and control BM and spleen cells were plated in methylcellulose media in the presence or absence of cytokines. (B) LSK cells, CMPs, GMPs, and MEPs from Ptpn11^D61Y and control mice were purified by FACS and plated in methylcellulose media in the absence of cytokines. (C) LSK cells, CMPs, and GMPs from ER-Cre and ER-Cre;Ptpn11^D61Y mice were purified and cultured for 16 hours in media containing SCF, LIF, IL-6, sIL-6R (for LSK), and SCF and IL-11 (for CMPs and GMPs) in the presence or the absence of Tam. Live cells were replated on methylcellulose medium in the absence of cytokine. For all experiments, colonies were counted after 7 to 9 days.