Sequence logos for alignments of selected subsets. In these subfamily logos,⁵⁰  each FR/CDR position is displayed as a stack of upright AA symbols. The height of each one-letter AA symbol is directly proportional to the relative frequency of that AA at a given FR/CDR position in the alignment among all sequences of a given subset. For clarity, the germline AAs of the IGKV or IGLV allele used by a given subset are shown upside down in gray color: the height of the inverted germline AA symbol is the sum of the heights of the upright AAs representing only the germline AA used by the cases that showed a change. Blank spaces represent AAs that are unchanged in the CLL IGKV or IGLV sequence compared with the germline sequence. For clarity, logos are vertically stretched so that the tallest upright stacks are of the same size, irrespective of its information content (ie, number of sequences). More information about number of sequences with a certain AA change of total number of sequences in each subset can be found in Table 2 and Table S21. AAs are divided in groups and color-coded as follows: GAPVLIM in blue, FYW in purple, STCNQ in green, KRH in red, and DE in orange. (A) Subset no. 2; (B) subset no. 4.