Both mRNA and protein levels of PDLIM2 were decreased in HTLV-I–transformed T cells. (A) Protein expression of PDLIM2 was analyzed in the indicated cell lines by IB. (B) CHX-chase assays were performed using 293 cells transfected with PDLIM2, PDLIM2/Tax, and HuT-102 cells stably expressing PDLIM2, followed by IB to detect PDLIM2 protein levels. (C) RNA expression of PDLIM2 was analyzed in the indicated cell lines by real-time PCR. The relative levels of PDLIM2 mRNA were normalized according to β-actin mRNA level and represented as percentile in Jurkat cells (arbitrarily set as 100). The data presented are the mean plus or minus standard deviation (n = 3).