SAA induces G-CSF secretion in monocytes and macrophages. Freshly prepared human PBMCs (A), mouse BMDMs (B), and mouse RAW264.7 cells (C) were stimulated with SAA (1 μM) or buffer (NS), and the secreted G-CSF was determined with ELISA at the indicated time points. Data shown are mean plus or minus SEM from 3 experiments.