Transcription factor binding and chromatin structure at the pb4.2, eklf, and gpa promoters in Ter119⁻ cells. (Top) Schematic representation of the promoter (P) and distant upstream (N) regions in pb4.2, eklf, and gpa loci that were tested for transcription factor binding and chromatin modification. Numbers indicate the position of the cis-elements relative to the transcription start site (accession numbers are pb4.2, AF019074; eklf, AF033102; gpa, M26385). ■ indicates Gata sites; ●, E-box motifs and nucleotide sequence. In brackets under each locus is indicated the fold reduction in gene expression in the mutant cells compared with wild-type cells, as shown in Figure 5. (Below) Real-time PCR analysis of chromatin derived from Ter119⁻ population purified from scl^Wt/Wt and scl^RER/RER fetal liver cultured cells and immunoprecipitated using the antibodies indicated on the left. A no antibody control is also shown. The y-axis represents enrichment over input DNA, normalized to a control sequence in the gapdh gene. On the x-axis are shown the sequences that were analyzed; promoter areas (P) and negative points designed 5′ of the promoter regions (N). Error bars correspond to plus or minus 1 SD from at least 3 independent experiments; *P < .01.