Allelic expression imbalance and mRNA levels of VKORC1 in human liver, left ventricular heart and B-lymphocytes. (A) Allelic expression imbalance (AEI) in liver. Genomic DNA and cDNA were amplified by PCR, and allelic mRNA and DNA ratios were measured in samples heterozygous for marker SNP (3730A>G, rs7294) located at 3′-UTR of VKORC1. The A/G ratio for DNA was set to 1, and ratios for cDNA were normalized to that of DNA. Three different conditions were used to measure AEI and the results were combined: E1, cDNA was amplified using primers within exon3; E2 and E3, complete cDNA was amplified. For AEI measurement in E1 and E2, the forward extension primer was used, and for E3, the reverse extension primer. (B) VKORC1 mRNA expression in 65 human liver samples grouped by −1639G>A (or 1173C>T) genotypes. (C) and (D) AEI in left ventricular heart and B-lymphocytes. Data are means (± SD), *P < .05 versus GG, #P < .05 versus GA, ANOVA with Dunnett post test.