IFN-γ treatment of Fanca^–/– and Fancg^–/– recipients is sufficient to allow engraftment of syngeneic WT bone marrow cells. (A) CD45.1⁺ WT BM nucleated cells (10⁷) were injected into the tail vein of Fanca^–/– and Fancg^–/– C57Bl/6 recipients that express the CD45.2 antigen. Recipients were pretreated with IFN-γ or vehicle control. The percentage of CD45.1⁺ cells in the peripheral blood was determined by fluorescence cytometry 6 months following transplantation. WT and Fancc^–/– mice treated with PBS or IFN-γ were used as controls. Data points represent CD45.1⁺ chimerism of individual mice. Bars represent the mean CD45.1⁺ chimerism. *P < .001 comparing chimerism of Fanca^–/– or Fancg^–/– recipients treated with IFN-γ versus vehicle-treated Fanca^–/– recipients and Fancg^–/– recipients. Multilineage analysis of donor CD45.1 cells in representative (B) Fanca^–/– and (C) Fancg^–/– mice. The percentage of WT CD45.1⁺ lymphoid (CD3 and B220) and myeloid (Gr1 and Mac1) cells is shown in the top right corner of each fluorescence-activated cell sorter (FACS) profile.