Smad7-overexpressing cells show increased repopulative capacity upon secondary transplantation. (A) FACS analysis of PB from secondary recipients 12 to 14 weeks after BM transplantation. The top panel shows Ly5.1/Ly5.2 analysis and the bottom panel shows GFP within the Ly5.1/5.2 population. Filled bars indicate MIG; open bars, Smad7. (B) Average percentage of total GFP⁺ cells in PB of secondary recipients 12 to 14 weeks after BM transplantation: MIG, 9.40% ± 4.95%; Smad7, 40.6% ± 3.45% (P = .003). All mean values are from 4 independent transductions ± SEM; a total of 78 secondary recipients were used.