Smad7-overexpressing cells can reconstitute lethally irradiated recipients long-term and generate a normal lineage distribution. (A) FACS analysis of PB from primary recipients at 16 to 18 weeks after BM transplantation. The top panel shows Ly5.1/Ly5.2 analysis and the bottom panel shows the GFP contribution within the Ly5.1/5.2 population. (B) Average percentage of total GFP⁺ cells in PB of primary recipients 16 to 18 weeks after BM transplantation: MIG, 25.6% ± 11.1%; Smad7, 36.9 ± 7.35%. (C) Lineage distribution within the GFP⁺ population in PB of primary recipients. B220, CD3, and Gr1/Mac1 indicate B cells, T cells, and myeloid cells, respectively. B220⁺ cells were represented on average 59.6% ± 4.95% for MIG and 59.6% ± 1.50% for Smad7, CD3⁺ cells 15.7% ± 1.15% for MIG and 15.2% ± 2.20% for Smad7, Gr1⁺/Mac1⁺ cells 18.5% ± 3.50% for MIG versus 18.0% ± 3.70% for Smad7. Data are pooled from 4 separate transduction experiments and a total of 24 recipients. Error bars indicate standard error of the mean (SEM).