PTPγ expression was increased by LPS and appeared to colocalize with MHC class II on DC plasma membrane. moDCs were left untreated (top panels) or were treated with LPS for 24 hours (bottom panels) and then were stained with control antibody (rabbit [Rbt] IgG), anti-PTPγ affinity-purified polyclonal antibody P4, followed by anti–Rbt IgG-Cy3 (red), FITC-conjugated anti–MHC class II, and anti-CD83 antibodies (green). Insets show the double staining of PTPγ-expressing cells (red) with MHC class II (third column) and CD83 (last column), both in green. PTPγ, MHC class II, and CD83 signals all increased at the cell surface on LPS treatment. MHC class II, and especially CD83, also appeared as a bright green spot in a perinuclear localization. Yellow indicates colocalization of the selected proteins that is particularly evident at the cell surface (inset, third column from left, bottom panel) corresponding to LPS-treated moDCs stained with anti–MHC class II and -PTPγ antibodies.