Phenotypic analysis of fresh CD56^bright CD16^– dNK cells. Mononuclear cells were derived from decidua or peripheral blood. (A-B) Cells were analyzed for CD16 and CD56 surface expression. In each sample, 10⁴ cells were acquired for FACS analysis. (C-F) Three-color immunofluorescence analysis with the indicated surface or intracellular markers was performed by gating on CD3^– CD56⁺ cells. Similar results were obtained in 20 different samples analyzed.