Phenotypic and functional properties of MoDCs from healthy donors (HD) and myeloma patients (MM). Representative histograms showing the expression of CD1a, CD40, CD80, and HLA-DR on (A) immature and (B) mature MoDCs generated from a healthy donor and a myeloma patient. Numbers inside the histograms represent the mean fluorescence intensity (MFI). (C) Pooled data depicting the expression (MFI) of CD1a, CD40, CD80, and HLA-DR on mature MoDCs from all tested healthy donors (n = 10) and myeloma patients (n = 12). Bars indicate the mean MFI. (D) Allostimulatory capacity of mature MoDCs in activating alloreactive T cells in an MLR assay. Shown are the mean ± SD of the results obtained with cells from 7 healthy donors and 7 myeloma patients. (E) Antigen-presentation capacity of MoDCs to autologous T cells. Shown are the mean ± SD of the results of cells presenting recalled antigen PPD to autologous T cells obtained from 5 healthy donors and 5 myeloma patients. In these experiments, different T cell/DC ratios (10:1, 100:1, and 1000:1; noted in the figure) were used. * P values less than .05.