IkL/L mice show selective loss of pDCs. (A) Splenocytes from the indicated mice were stained with Alexa488 anti-120G8; PE anti-CD3, CD19, and NK1.1; APC anti-CD11c; PE-Cy7 anti-CD11b; APC-Cy5.5 anti-B220; and biotin anti-CD8α. Anti-CD8α antibodies were revealed with SA-PE-TexasRed. The resulting cells were analyzed on an LSR II flow cytometer by collecting 200 000 events for each file. Nonviable cells were eliminated from subsequent analyses by staining cells with DAPI. Numbers indicate percentage of events within the indicated gate as a function of the corresponding parent gate for each plot. The absolute number of events within each CD11c⁺ (CD3^– CD19^– NK1.1^–) gate are as follows: WT, 889; Ik^+/L, 795; and Ik^L/L, 1105. (B) Lymph node cells from the identical mice were stained and analyzed as in panel A. The absolute number of events within each CD11c⁺ (CD3^– CD19^– NK1.1^–) gate are as follows: WT, 237; Ik^+/L, 241; and Ik^L/L, 164. Absolute numbers of DCs within each subset in 3 mice per group are given in Table 1. These data are representative of 4 experiments.