CDC42GAP regulates MAPK signaling in neutrophils. (A-B) Neutrophils were prestimulated with fMLP and seeded on fibrinogen for the indicated time. The cells were lysed and analyzed for phosphorylated p42/44 followed by total p42/44 (A) and for phosphorylated p38 followed by total p38 (B). (C) Neutrophils were stimulated with fMLP alone for the indicated time and examined for phosphorylated p42/44 and phosphorylated p38. Representative blot from 2 to 3 independent experiments is shown.