Loss of CDC42GAP functions is associated with abnormal assembly of podosome-like structures and filopodia. F-actin assembly and organization of adhesion structures. (A) Neutrophils were prestimulated with 10 μM fMLP and seeded on fibrinogen-coated slides for 30 minutes. The cells were then fixed and stained with rhodamine-phalloidin (in red). Figure S1 shows more representative pictures. Two representative images of each genotype from 3 independent experiments are shown. (B) Neutrophils were prestimulated with fMLP and seeded on fibrinogen-coated slides for 30 minutes. The cells were then fixed and stained with rhodamine-phalloidin (in red) and antivinculin followed by anti–mouse Alexa488 (in green). Two representative images of each genotype from 3 independent experiments are shown. (C) The percentage of cells displaying more than 1 membrane protrusion and increased filopodia was quantified. (D) The percentage of cells displaying podosome-like structures was quantified. All histograms represent the mean ± SD from 3 independent experiments.