Tissue microarray-based p-AKT analysis in DLBCL patients. (A) Overview of the TMA containing 100 DLBCL tissue samples. (B) Kaplan-Meier survival curve of DLBCL of p-AKT expression by immunohistochemistry. (C) Immunohistochemistry of a tissue spot showing high p-AKT expression in DLBCL tumor (objective, 20×/0.70 NA). (D) A tissue spot showing negative p-AKT expression (objective, 20×/0.70 NA). Immunohistochemical staining images were obtained with a BX51 Olympus microscope and an Olympus DP12 camera (Olympus, Melville, NY). Images were viewed through a universal semi-apochromat objective lens (UPlan F1; Olympus America, Woodbury, NY). Magnifcation, 200×.