LY294002-induced apoptosis in DLBCL cell lines. (A) SUDHL-4, SUDHL-5, SUDHL-8, SUDHL-10, and OCI-LY19 cell lines were treated with 10 and 25 μM LY294002 for 24 hours and cells were subsequently stained with fluorescein-conjugated annexin V antibody and propidium iodide (PI) and the ratio of apoptotic cells was analyzed by flow cytometry. (B) SUDHL-4, SUDHL-8, SUDHL-10, and OCI-LY19 cells were treated with various doses of LY294002 for 24 hours and apoptosis was determined using TUNEL assays. (C) SUDHL-4 and OCI-LY19 cells were treated with 25 μM LY294002 for 24 hours and DNA was extracted and separated by electrophoresis on 1.5% agarose gel.