Effect of LY294002 treatment on cell death in DLBCL cell lines. (A) Dose-response curves to compare IC₅₀ of LY294002 in DLBCL cell lines. SUDHL4, SUDHL8, and OCI-LY19 cells were treated with various doses (5-100 μM) of LY294002 for 24 hours. Cell death was measured and IC₅₀ was determined by trypan blue exclusion assay as described in “Patients, materials, and methods.” (B) LY294002 inhibits cell viability in DLBCL cell lines. SUDHL-4, SUDHL-5, SUDHL-8, SUDHL-10, and OCI-LY19 cells were treated with 10 and 25 μM LY294002 for 24 hours. Percentage cell death was scored using trypan blue exclusion dye. The graph displays the mean ± SD of 3 independent experiments. *P < .001, statistically significant (Student t test). (C). LY294002 treatment increases sub-G₁ (Apo) population in DLBCL cells. SUDHL-4, SUDHL-5, SUDHL-8, SUDHL-10, and OCI-LY19 cells were treated with 10 and 25 μM LY294002 for 24 hours. Thereafter, the cells were washed, stained with propidium iodide, and analyzed for DNA content by flow cytometry as described in “Patients, materials, and methods.”