Sty I RFLP for the identification of the GATA mutation (−46 T → C). DNA was amplified using FY1 and FY2 primers11 for the amplification of a DARC fragment encompassing nt −46. The restriction fragments were separated on 12% acrylamide gel. (A) Schematic diagram of fragments generated bySty I digestion of the DARC fragment encompassing nt −46 FY*B, GATA mutation. (B) RFLP patterns of DNA from samples with the indicated phenotyes, identified by antisera, and genotypes, as determined by Ban I (the 12-bp fragment is not detected in this gel). Lanes: 1, 2, and 4, Fy (a+b−)FY*A/FY*A; 3 and 7, Fy(a-b-)FY*B/FY*B; 5 and 6, Fy (a+b−)FY*A/FY*B.