Morphological assessment of neutrophil apoptosis after 12-hour culture. (A) shows cytospins, prepared as described in Materials and Methods. Nonapoptotic neutrophils with multilobed nuclei are clearly distinguishable from apoptotic neutrophils which have smaller, denser nuclei and decreased cell volume. (B) shows summary data of apoptosis by morphology, and the effects of various agents added to neutrophil suspensions, as described in Materials and Methods. * Indicates values that are significantly different from controls (P = .01) after at least three separate experiments.