Depletion of FAA from the nucleus abolishes its ability to complement MMC hypersensitivity in FA-A lymphoblasts. (A) Schematic representation of FAA-NES and FAA-P12. The double-headed arrow indicates the amino acid substitution leading to inactivation of the NES. (B) (see page 2232) Immunofluorescence microscopy using pcDNAFAA-NES in transiently transfected Hela cells. (C) Western blot showing the stable expression of FAA, FAA-NES, and FAA-P12 in HSC72 cells. (D) MMC sensitivity assays of the HSC72-derived stable transfectants. (□), Control; (▪), FAA; (○), FAA-P12; (•), FAA-NES. A representative experiment is shown.