TCRV_β DNA sequences of patients A through C.

Panels A, B, and C show the sequencing results of patients A, B, and C, respectively. DNA sequences were derived from direct sequencing of the CD57⁺ PCR products from patient A (labeled Gel-A1 in panel A) and patient C (labeled Gel-C in panel C). No readable sequence was obtained from direct sequencing of the CD57⁻ PCR products, except in the case of patient A (labeled Gel-A2 in panel A). Plasmids B-1 to B-6 are 6 independent sequences obtained from 6 cDNA clones chosen at random from a library containing the CD57⁺ PCR product from patient B. Plasmids B-7 to B-11 are the independent sequences obtained from 5 cDNA clones from the CD57⁻ PCR product from the patient shown in panel B. Plasmids C-1 to C-8 are 8 independent sequences obtained from 8 cDNA clones chosen at random from a library containing the CD57⁻ PCR product from patient C (panel C). The sequences summarized in this Figure have been given the European Molecular Biology Laboratory (Heidelberg, Germany) nucleotide sequence database accession numbers: AJ276183 through AJ276184 for panel A, AJ276185 through AJ276195 for panel B, and AJ276196 throughAJ276204 for panel C. Keys: … identical nucleotides; ∼ ∼ ∼ absence of an amino acid at the relative position in the CDR3 sequence alignment.