TCRV_β CDR3 length analysis.

The PCR products (approximately 220 base pairs) and molecular markers were run on a 6% polyacrylamide gel, and the sizes of the radioactive PCR bands were determined by exposing an x-ray film to the gel. Expanded T cells were sorted according to their specific surface phenotype of TCRV_β⁺ and CD8⁺ and CD57 expression. Bands are lined up side by side for ease of comparison. The relative position of the PCR products of different TCRV_β families to each other is not the same as the actual gel. *Expanded TCRV_β populations are underlined. (A) CDR3 length analysis results from CD8⁺ T-cell expansions found in 6 patients with MM, together with CD8 and CD57 expression data. (B) Results of 6 age-matched normal controls for comparison. **Direct DNA sequencing has been performed on some of the PCR products and results are summarized.