STI571 alters the intensity of protein tyrosine phosphorylation in primary hematopoietic cells from mice with the CML-like illness.

Protein lysates from peripheral blood granulocytes (B) or BM (M) from the indicated placebo (control, lanes 3-6) and STI571-treated (lanes 7-11) mice were analyzed by antiphosphotyrosine immunoblot. A Ba/F3 cell line transduced with P210 (B/A) or retroviral vector alone (N) is shown at left as a control. The positions of P210^BCR/ABL, Shc, and Crk-L are shown at far left.