Effect of IL-7 and IL-15 on the DNA-binding of c-Myb in SeAx cells.

(A) Nuclear extracts from different cell lines were incubated with a radioactively labeled oligonucleotide containing the c-mybbinding site of the bcl-2 promoter. Lanes 1 to 3, no interleukin added. Nuclear extracts from HUT78 (lane 1), MyLa 2059 (lane 2), and SeAx cells (lane 3). Lane 4, SeAx cells treated with 10 U IL-7. Lane 5, SeAx cells treated with 10 U IL-15. Lane 6, SeAx cells treated with 10 U IL-7 and IL-15. Lane 7, nuclear extract from PBLs of a healthy donor. (B) The binding of c-Myb to its target sequence is inhibited by a c-Myb antibody. Lanes 1 to 6, no antibody added. Lanes 6 to 10, c-Myb antibody added. Lanes 1 and 6, nuclear extract from PBLs. Lanes 2 and 7, nuclear extract from HeLa cells. Lanes 3 and 8, nuclear extract from HUT78 cells. Lanes 4 and 9, nuclear extract from MyLa 2059 cells. Lanes 5 and 10, nuclear extract from SeAx cells. Lanes 11 and 12, STAT5 antibody added as negative control. Lane 11, nuclear extract from HUT78 cells. Lane 12, nuclear extract from MyLa 2059.