Fig. 7.
Effect of β-GMR and β-GMRIT on α/β-GMR–mediated cell survival.
The α/β-GMR–mediated cell survival is supported by β-GMR but inhibited by β-GMRIT. BaF-3/α/β-GMR (top), BaF-3/α/β-GMR plus β-GMRIT (clone no. 3, middle), and BaF-3/α/β-GMR plus β-GMR cells (bottom) were grown in 0.2 (left), 2 (middle), or 20 ng/mL huGM-CSF (right). Cells were incubated with PI and annexin V after 48 hours and analyzed by FACS. Annexin V–positive cells were considered apoptotic. When the cells were cultured in WEHI-CM, the rate of apoptosis was equally low (less than 6%) in all clones tested. Data represent 1 out of 3 experiments.
