Protein expression of α/β-GMR, β-GMR, and β-GMRIT in transfected BaF-3 cells.
(A) Immunoprecipitates of β-GMRIT from BaF-3/α/β-GMR (left) and BaF-3/α/β-GMR plus β-GMRIT cells (clones no. 1 through 5) were immunoblotted with a monoclonal antibody against the extracellular domain of β-GMR and β-GMRIT. The molecular weight is given in kilodaltons on the left, and the migration of β-GMRIT is indicated by the arrow. (B) BaF-3/α/β-GMR (top), BaF-3/α/β-GMR plus β-GMRIT(no. 3, middle), and BaF-3/α/β-GMR plus β-GMR (bottom) cells were stained with an anti–α-GMR antibody recognizing α/β-GMR (black) and an anti–β-GMR antibody recognizing both β-GMR variants (gray) followed by FACS analysis. The interrupted line represents controls stained with the secondary antibody only.
