Fig. 3.
The β-GMRIT inhibits cell survival mediated by α-GMR plus β-GMR.
BaF-3/α-GMR plus β-GMR (upper panels) and BaF-3/α-GMR plus β-GMR plus β-GMRIT cells (no. 1, lower panels) were grown in either low (2 ng/mL, left) or high (20 ng/mL, right) concentrations of huGM-CSF. Cells were incubated with PI and annexin V after 48 hours and analyzed by FACS. Annexin V–positive cells were considered apoptotic. When the cells were cultured in WEHI-CM, the rate of apoptosis was equal in all clones tested.
