mRNA and protein expression of GMR-constructs in transfected BaF-3 cells.
The mRNA expression of GMR-constructs in BaF-3/α-GMR plus β-GMR cells after cotransfection of β-GMRIT. Primer set A was used for amplification of α-GMR by conventional RT-PCR as described in “Materials and methods.” The β-GMR and β-GMRITwere detected with primer set C, resulting in amplicons of 386 bp and 282 bp, respectively. The α plus β indicates BaF-3/α-GMR plus β-GMR cells, and Nos. 1 through 4 indicate independent BaF-3/α-GMR plus β-GMR plus β-GMRIT clones. (B) Surface expression of GMR-constructs in transfected BaF-3 cells. BaF-3/α-GMR plus β-GMR (panel Bi) and BaF-3/α-GMR plus β-GMR plus β-GMRIT cells (panel Bii) were stained with an anti–α-GMR (black) and an anti–β-GMR antibody recognizing both isoforms of β-GMR (gray) followed by FACS analysis. The interrupted line represents controls stained with the secondary antibody only.
