Fig. 4.
Fig. 4. Chemotactic responses to TECK/CCL25. / Freshly isolated thymocytes from CCR9-deficient (CCR9−/−), CCR9-heterozygous (CCR9+/−) and wild-type mice were placed in the upper chamber of a 24-well Transwell plate. Recombinant TECK/CCL25 (200 nM) or culture medium, as a negative control, were placed in the lower chamber. After 4 hours of incubation, cells in the lower chamber were counted both manually and by flow cytometry.

Chemotactic responses to TECK/CCL25.

Freshly isolated thymocytes from CCR9-deficient (CCR9−/−), CCR9-heterozygous (CCR9+/−) and wild-type mice were placed in the upper chamber of a 24-well Transwell plate. Recombinant TECK/CCL25 (200 nM) or culture medium, as a negative control, were placed in the lower chamber. After 4 hours of incubation, cells in the lower chamber were counted both manually and by flow cytometry.

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