Analysis of the chemokine receptor transcripts expressed in freshly isolated thymocytes.

Total RNA extracted from thymi and spleen from CCR9-deficient and wild-type mice were analyzed by multiprobe ribonuclease protection assay using a mCR-5 RiboQuant mouse template set (BD Pharmingen). The autoradiogram shows the mCR-5–probe set not treated with RNases (lane 1), and the RNase-protected bands following hybridization with control yeast tRNA (line 2) with RNA isolated from CCR9-deficient (lane 3), or wild-type thymi (lane 4). Also shown are the RNase-protected bands obtained after hybridization with RNA isolated from CCR9-deficient spleens (lane 5), or wild-type spleens (lane 6). The identity of the various protected bands is indicated on the left.