Quantitation of B-CLL apoptosis by flow cytometric analysis.

Percentage of apoptotic B-CLL cells was determined by flow cytometric quantitation of the proportion of cells with a subdiploid DNA content (A) or changes in light-scattering properties (B). Incubations were carried out in the absence (open bars) or presence (hatched bars) of 50 ng/mL anti-Fas IgM.