Semiquantitative RT-PCR analysis of B-CLL transcripts.

(A) Analysis of actin (A), Fas (F), or Fas-L (FL) transcripts in B-CLL cells at day 0 (D0) or after 24-hour incubation with medium alone (control), 165 μM chlorambucil (Chl), 17.5 μM fludarabine (Flu), or 24 hours after irradiation (10 Gy). (B, C) Intensities of Fas, Fas-L, and actin PCR bands from multiple B-CLL samples were quantified. Fas/Actin and Fas-L/Actin ratios after treatment were compared to 24-hour controls and are presented in panels B and C, respectively.