Fig. 7.
Adipocyte differentiation from MPCs.
MPCs were cultured at 2 × 104/cm2 in expansion medium without FCS, EGF, and PDGF-BB, but with 10% horse serum. Adipocyte differentiation was shown by oil-red staining (A; enlargement = 20 ×) and presence of PPAR-γ (B, clone no. 3; see also Figure 12; β-actin serves as loading control) on day 14. A representative example of more than 5 experiments is shown.
