Fig. 3.
Fig. 3. Characteristics of culture-expanded MPCs. / Culture-expanded MPCs have long telomeres and are cytogenetically stable. (A) CD45−GlyA− cells from 4 donors ages 2 to 50 were plated on FN in expansion medium with 2% FCS, 10 ng/mL EGF, and 10 ng/mL PDGF-BB, and passaged for 35 cell doublings. Cells were harvested and telomere lengths evaluated. Lanes 1 = 22 years; lane 2 = 35 years; lane 3 = 50 years; lane 4 = 2 years; lane 5 = 293 cells; lane 6 = HL60 cells. ATL indicates average telomere length. (B) CD45−GlyA− cells were plated on FN in expansion medium with 2% FCS, 10 ng/mL EGF, and 10 ng/mL PDGF-BB and passaged for 40 cell doublings. Cells were harvested 24 hours after replating and were cytogenetically analyzed. A representative example of 4 experiments is shown.

Characteristics of culture-expanded MPCs.

Culture-expanded MPCs have long telomeres and are cytogenetically stable. (A) CD45−GlyA− cells from 4 donors ages 2 to 50 were plated on FN in expansion medium with 2% FCS, 10 ng/mL EGF, and 10 ng/mL PDGF-BB, and passaged for 35 cell doublings. Cells were harvested and telomere lengths evaluated. Lanes 1 = 22 years; lane 2 = 35 years; lane 3 = 50 years; lane 4 = 2 years; lane 5 = 293 cells; lane 6 = HL60 cells. ATL indicates average telomere length. (B) CD45−GlyA− cells were plated on FN in expansion medium with 2% FCS, 10 ng/mL EGF, and 10 ng/mL PDGF-BB and passaged for 40 cell doublings. Cells were harvested 24 hours after replating and were cytogenetically analyzed. A representative example of 4 experiments is shown.

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