Fig. 9.
Fig. 9. DMS- and staurosporine-induced cyt c and Smac/DIABLO release and caspase-3 processing are blocked in Bcl-xL–overexpressing Jurkat cells. / (A) Cytosolic extracts from Jurkat/neo or Jurkat/Bcl-xLcells treated with 20 μM DMS or 500 nM staurosporine for 5 hours were subjected to 15% SDS-PAGE and immunoblotted with anti-cytc, anti-Smac/DIABLO, and anti-COX II, as described in Figure1. (B) Proteins from duplicate extracts were analyzed by immunoblotting with anti–caspase-3 antibody. The mobilities of the 32-kd precursor (p32) and proteolytically processed forms (p20 and p17) are indicated. Similar results were obtained in 3 independent experiments.

DMS- and staurosporine-induced cyt c and Smac/DIABLO release and caspase-3 processing are blocked in Bcl-xL–overexpressing Jurkat cells.

(A) Cytosolic extracts from Jurkat/neo or Jurkat/Bcl-xLcells treated with 20 μM DMS or 500 nM staurosporine for 5 hours were subjected to 15% SDS-PAGE and immunoblotted with anti-cytc, anti-Smac/DIABLO, and anti-COX II, as described in Figure1. (B) Proteins from duplicate extracts were analyzed by immunoblotting with anti–caspase-3 antibody. The mobilities of the 32-kd precursor (p32) and proteolytically processed forms (p20 and p17) are indicated. Similar results were obtained in 3 independent experiments.

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