Fig. 8.
DMS potentiates cyt c and Smac/DIABLO release and caspase-3 activation induced by ceramide.
Jurkat (A) or U937 cells (B) were preincubated with 5 μM DMS for 15 minutes and then treated for an additional 5 hours without or with 25 μM C6-ceramide. Cytosolic extracts were subjected to 15% SDS-PAGE and immunoblotted with anti-cyt c, anti-Smac/DIABLO, and anti-COX II, as described in Figure 1. Caspase-3–like activity in duplicate extracts from Jurkat (C) or U937 cells (D) was measured with the fluorogenic substrate Ac-DEVD-AMC. Results are means of 3 independent experiments.
