DMS-induced cyt c and Smac/DIABLO release is suppressed by cotreatment with S-1P.

Cytosolic extracts from Jurkat (A), U937 (B), or HL-60 cells (C) treated for 5 hours (A, B) or 8 hours (C) with 20 μM DMS in the presence or absence of 5 μM S-1P were subjected to 15% SDS-PAGE and immunoblotted with anti-cyt c, anti-Smac/DIABLO, and anti-COX II, as described in Figure 1. Similar results were obtained in 3 independent experiments.