Flow cytometry analyses of human hematopoietic cells engrafted in NOD-SCID mice.

Six weeks after injection in a control animal injected with CD34⁺ cells transduced with the LZRS-EGFP retroviral vector (A), CD19⁺ cells are more abundant than CD33⁺cells and both populations express significant levels of the EGFP marker. The same pattern is observed in one of the test animals injected with the LZRS-Ik6-EGFP retroviral vector (B). In another test animal injected with CD34⁺ cells transduced with the LZRS-Ik6-EGFP retroviral vector (C), a different pattern is seen. A small percentage of cells expressing CD19 can be identified; only rare CD19⁺ cells express EGFP, in contrast with CD33⁺ cells recovered from the same animal. Similar numbers of events were acquired during flow cytometry analysis for all lines. For detailed analyses, see Table 2.