Comparison of lymphoid or myeloid antigen expression in transduced cells after in vitro differentiation.

Percentage of cells positive for CD10 or CD19 (B lineage) or CD14 or CD33 (myeloid lineage) in the “lymphocyte” gate of 6- to 8-week-old MS-5 cocultures, initiated with sorted EGFP⁺ placental CD34⁺ cells, transduced with LZRS-Ik6-EGFP or LZRS-EGFP (paired experiments). Five of 6 experiments showed a decrease of CD10⁺ or CD19⁺ cells and an increase of CD14⁺ and CD33⁺ cells in cultures initiated with CD34⁺ cells transduced with LZRS-Ik6-EGFP when compared with cultures initiated with LZRS-EGFP transduced CD34⁺ cells.