Induction of TRAP-positive cells by RANKL and FL in spleen cells from op/op mice.

Spleen cells were incubated for 24 hours in FL (10 ng/mL). Nonadherent cells were then incubated as shown for 6 days, and the cells formed were assessed for expression of TRAP. Results are expressed as mean ± SEM of 12 cultures per variable. *P < .05 versus cultures containing no FL.