Expression of the Gr B, gp49B, ST2L, TPH, and HDC genes in +/+ and mi/mi CMCs. Five micrograms of total RNA prepared from +/+ or mi/mi CMCs was loaded in each lane and fixed onto nylon membranes by capillary action. The membranes were hybridized with specific DNA probes. To prepare the Gr B, gp49B, ST2L, TPH probes, the cDNA inserts of clones no. 11, 232, 239, and 382 were used. Expression of MMCP-6 and MC-CPA mRNA were shown as controls. Arrows indicate the specific signals which are accompanied by their molecular size. Arrowheads indicate the position of 18S and 28S in each panel. Reprobing with the β-actin probe allowed verification that an equal amount of mRNA was loaded per lane.