Fig. 7.
![Fig. 7. Absence of Nrf-2 protein in Nrf-2 (-/-) embryonic stem (ES) cells. (A) Immunoprecipitation of [35S]-labeled Nrf-2 from in vitro transcription/translation reaction showing the starting material, immunoprecipitation with preimmune serum, and immunoprecipitation with anti-Nrf2 antibody. The 98-kD marker is indicated. Nrf-2 migrates at 96 kD.26 (B) Immunoprecipitation of [35S]-labeled Nrf-2 from whole cell extracts of 1 × 107 COS cells transfected with vector alone, or with Nrf-2 cDNA in COS expression vector pEUK-1 (Clontech). (C) Immunoprecipitation of [35S]-labeled Nrf-2 from whole cell extracts of 4 × 107 Nrf-2 (+/+) ES cells and Nrf-2 (-/-) ES cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/9/10.1182_blood.v91.9.3459/3/blod40940007w.jpeg?Expires=1722710106&Signature=ejQogAdDGATa8Bz~aplJ5gaDO78Rd1w~8rClocp-b62TVOFp-bRFAphLwkWrl9eLFU1Q06e5RXZMhP5XYbJcRVWHB2xKKpkWieQ5HiOzQnKIGaUJ7cp5jMe-W4BH3EVJJbrLVUXwHmIFbhRfv3B8oR1I6Tr1dBEGa9RZmcxUbv9F600zSy3rJP-XrNbMWN9iN780D2~t9N8tcVKKpV33tJDwEI3HuEWnp-prg3XwOcBiV7oan5wp1nHIwmeg5GS8-roFXJwfqFtfbrZP4MbwJOd1stmD6QgH~liyFzViQfSjgD5MNPHNhuLAECFEX84WRePNrZI0fh8nXMTdcijcMQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Absence of Nrf-2 protein in Nrf-2 (-/-) embryonic stem (ES) cells. (A) Immunoprecipitation of [35S]-labeled Nrf-2 from in vitro transcription/translation reaction showing the starting material, immunoprecipitation with preimmune serum, and immunoprecipitation with anti-Nrf2 antibody. The 98-kD marker is indicated. Nrf-2 migrates at 96 kD.26 (B) Immunoprecipitation of [35S]-labeled Nrf-2 from whole cell extracts of 1 × 107 COS cells transfected with vector alone, or with Nrf-2 cDNA in COS expression vector pEUK-1 (Clontech). (C) Immunoprecipitation of [35S]-labeled Nrf-2 from whole cell extracts of 4 × 107 Nrf-2 (+/+) ES cells and Nrf-2 (-/-) ES cells.
