American Society of Hematology

Fig. 5.

$Fig. 5. PKH26 staining of purified candidate HSC before and after culture in SCF + IL-3 + FL. (A) PKH26 was incorporated into the lipid bilayer of purified candidate HSC at day 0. On day 8, PKH26 intensity was analyzed again and cells were sorted into PKHlo (gate1) and PKHhigh fractions (gate2) for TRAP. The cells in the PKHlo fraction have undergone several rounds of division, resulting in diminished dye fluorescence. (B) Telomerase activity in PKHhi and PKHlocells. PKHhi and PKHlo cells were sorted from BM1 after 8 days of culturing SCC in SCF + FL + IL-3. TRAP assay was performed on CHAPS extracts equivalent to 1,000 cells in the absence (−) and presence (+) of RNase A.$

PKH26 staining of purified candidate HSC before and after culture in SCF + IL-3 + FL. (A) PKH26 was incorporated into the lipid bilayer of purified candidate HSC at day 0. On day 8, PKH26 intensity was analyzed again and cells were sorted into PKH^lo (gate1) and PKH^high fractions (gate2) for TRAP. The cells in the PKH^lo fraction have undergone several rounds of division, resulting in diminished dye fluorescence. (B) Telomerase activity in PKH^hi and PKH^locells. PKH^hi and PKH^lo cells were sorted from BM1 after 8 days of culturing SCC in SCF + FL + IL-3. TRAP assay was performed on CHAPS extracts equivalent to 1,000 cells in the absence (−) and presence (+) of RNase A.

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