Phenotypic analysis of candidate HSC after culture. Candidate HSC were cultured for 5 days in (A) SCF, (B) FL, (C) IL-3, (D) SCF + FL, (E) SCF + IL-3, (F) FL + IL-3, and (G) SCF + FL + IL-3. After 5 days, cells were stained with antibodies against CD34, CD45RA, and CD71. Profiles shown were from events gated for low PI, low SCC, and high expression of CD34. In (G) boxes I, II, and III represent the windows used to sort for CD34⁺CD45RA^loCD71^lo cells, CD34⁺CD45RA^loCD71^hi cells, and CD34⁺CD45RA^hiCD71^hi cells, respectively. The same windows were applied to Fig 4 and Table 1.