Anti–BCR-ABL AS-ODNs restore inhibition of CML CFC proliferation after coculture with stroma or FN. A total of 10,000 DR⁺ cells were incubated in serum-free medium for 96 hours with or without addition of breakpoint-specific and control ODNs. After incubation, cells were either kept in suspension (A) or cocultured with stroma (B); FN 75-kD (C); FN 33-66-kD (D); or poly-L-lysine (E) coated wells for 4 hours, after which proliferation was evaluated using thymidine suicide assays as described in the text. For stroma (B), results for adherent (hatched bars), nonadherent (open bars), and total (adherent + nonadherent) cells (solid bars) are shown separately. The significance values in (B) represent differences in proliferation of total (adherent + nonadherent) cells. Results represent the mean ± SEM for six separate experiments.